London, England, United Kingdom
Highly committed and motivated Biochemistry graduate, actively seeking work experience and opportunities for professional development in academic research and the pharmaceutical industry.
Experimental and Translational Medicine
Final year research student at Osier Lab - Imperial College London (77%) - Expression of Plasmodium falciparum recombinant proteins in an Expi293F mammalian system - Protein purification and characterisation - Characterised antibody-dependent effector mechanisms with large sample sets
- Managed an annual budget of ~£10,000 - Monitored society finances, including budgeting and expenses approvals. - Planned large-scale events to optimise running costs and monitor profits - Formulated sponsorship proposals and liaised with multiple stakeholders such as sponsors and event venues
Characterising molecular interactions between HIV-1 Vpr and host cell targets, supervised by Dr. N Matheson. - Designed chimeric proteins to identify key interaction domains and related downstream effects - Critically analysed results to hypothesise and design complementary validation experiments Key laboratory skills: - HEK293T and CEM-T4 cell culture and lentiviral transduction, Hi-Fi and Gibson Assembly cloning - Intracellular flow cytometry, co-immunoprecipitation and pull-down assays
Placement Project: Quantitative Analytical Methods for mRNA Characterisation (71%) - Evaluated the significance and application of current mRNA integrity characterisation methods - Investigated the effect of freeze-thaw cycles and storage temperature on the integrity of mRNA - Developed plate-based assays for detecting sample impurities with 10-fold higher sensitivity - Reviewed developments in mRNA therapeutics and the demand on corresponding analytical techniques - Presented findings and conclusions to the team Laboratory Skills: - Analysed mAbs, RNA and AAV using advanced techniques such as U/HPLC, CE and BLI - Troubleshoot assays including DNA extraction, ddPCR, RT-qPCR and cDNA synthesis - Designed and validated AAV analysis methods using mass photometry and DLS Experience: - Adhered to SOPs and SRAs under ISO9001 standards, while updating electronic lab notebooks - Gained valuable insight into budgeting and risk management through project meetings - Collaborated with multidisciplinary teams to prioritize and deliver project goals under tight timelines - Summarised training documents entailing general lab safety, assay and data analysis protocols