Halle, Saxony-Anhalt, Germany
Passionate researcher with 14+ years of experience. Dynamic and versatile research scientist with extensive detailed research and experimental experience. Holds a broad set of analytical and technical skills that would benefit any organization. Proven history of collaboration with cross-functional teams to achieve continuous improvement and far reaching objective. Expertise in many areas of research that utilize in vivo, ex vivo and in vitro skills and abilities. Pragmatic strategist able to focus on key issues and results to meet organizational priorities and make the necessary critical decisions to see them through. Proven ability to manage challenging research schedule while working in complex and highly-demanding environment. Can be reached via email: [email protected]; Tel: +49-1638973955
Here at the Institute for Physiological Chemistry, I am working on the generation and characterization of a new constitutively active mouse model for a transcriptional cofactor MRTF-A. MRTF-A acts a cofactor for SRF, thereby regulating a subset of the genes regulated by SRF. We believe that this mouse model would be proven useful in the study of tissue fibrosis of various organ as well as in the study of cancer metastasis.
As a post doctoral fellow I continued to work in the gastrointestinal physiology and developed in vivo methods to measure different physiological parameters. I tried to adopt and refine published in vivo methods to study transport across the gastrointestinal epithelium, as close as to the physiological situations. I measured bicarbonate and fluid transport in luminally perfused mice that were defective for ion transporters, the NHERF proteins, or for other regulatory proteins such as kinases, enzymes or receptors, under a variety of challenging experimental conditions. I also develop the fluorimetric method to assess intra as well as luminal surface pH in different segments of gastrointestinal tract using two-photon microscope in vivo. During this time I also standardized many immunohistochemistry protocols as well as methods to assess the mucus secretion in vivo.
Dissertation Title: "Duodenal mucosal bicarbonate secretion: role of CFTR, Slc26a6, carbonic anhydrase (II) and NHERF family adapter proteins in health an disease" During my PhD I established many techniques in the lab, collaborated and co-authored several conferences and international papers with researchers in different areas of gastrointestinal physiology, cell and molecular biology and cancer biology. My doctoral dissertation was conducted in the area of the duodenal mucosal bicarbonate secretion, electrolyte and fluid balance in relation to inflammatory bowl disease mouse models and mouse models for other disease such as cystic fibrosis. My recent research focused on the validation of formation of macromolecular signaling complexes in vivo with particular interest in cystic fibrosis, which will eventually lead to better understand the disease and therapeutic interventions. The development of in vivo setup for the measurement of various physiological parameters and in vivo two photon microscopy for the measurement of intra and extracellular pH, intracellular Ca2+ and mucus secretion measurements in the gut are major highlights of my research work so far.
I developed method to measure melanin pigment in different skin samples. The project involved enzymatic degradation of melanin followed by thin layer chromatographic separation of the products and estimation of melanin content using LC-MS.