Germany
I am a Cell Biologist (Dr. sc. nat., University of Zurich) with more than 9 years of research experience in cell biology, live-cell imaging and assay development. Background in applied maths and physics. My research focused on the development and optimization of cell-based assays, quantitative fluorescence microscopy and image analysis. I have worked with mammalian cell culture, molecular biology techniques, confocal and super-resolution microscopy, quantitative data analysis using Fiji/ImageJ, Excel, GraphPad, OriginLab and Python. During my postdoctoral research at the University Hospital Münster, I developed and optimized a live-cell imaging assay for quantitative analysis of Ca²⁺ oscillations in human sperm cells, including sample preparation, microscopy optimization and semi-automated image analysis. During my PhD at the University of Zurich, I established imaging workflows for quantitative colocalization studies of lipoproteins, SR-BI receptor variants and intracellular organelle markers using confocal, spinning-disk, SIM and STORM microscopy. I optimized sample preparation, staining and imaging protocols, analyzed large microscopy datasets. Earlier, I worked on recombinant GPCR membrane proteins at the Moscow Institute of Physics and Technology, where I gained experience in molecular cloning, protein expression, purification and characterization. I recently completed GMP training (160 hours), covering SOP development, CAPA, FMEA and quality management principles. I am looking for opportunities in the biotechnology and pharmaceutical industry where I can apply my experience in cell biology, assay development, quantitative imaging and molecular biology. I am open to relocation within Germany. Based in Germany. German language skills: B2. Permanent residence permit with unrestricted work authorization. Open to relocation within Germany. Deutsch: B2 (fortgeschritten). Standort Deutschland, uneingeschränkte Arbeitserlaubnis vorhanden.
Development and optimization of quantitative cell-based assays and live-cell imaging workflows. Key contributions: • Developed a quantitative live-cell imaging assay to analyze Ca²⁺ oscillations in sperm cells. • Established a reproducible workflow covering sample preparation, real-time imaging, experimental optimization and quantitative analysis. • Optimized imaging parameters including temperature control, focus stability and signal quality. • Developed a semi-automated image analysis workflow using Fiji/ImageJ and Python. • Performed quantitative analysis of fluorescence time-series datasets to identify dynamic cellular signaling patterns. • Improved assay robustness and reproducibility through systematic optimization of experimental parameters.
Research focused on quantitative cell biology, receptor trafficking and advanced fluorescence microscopy. Key contributions: • Developed and optimized cell-based assays investigating lipoprotein–cell interactions and intracellular transport processes. • Established quantitative imaging workflows for receptor localization and organelle trafficking analysis. • Performed systematic colocalization studies of SR-BI receptor variants, lipoproteins and intracellular organelle markers. • Applied advanced microscopy techniques including confocal microscopy, spinning-disk microscopy, SIM and STORM. • Developed reproducible image analysis workflows using Fiji/ImageJ for quantitative evaluation of complex microscopy datasets. • Optimized cell culture, staining and imaging protocols to improve data quality and reproducibility. • Supervised students and collaborated with microscopy specialists and interdisciplinary research teams.
Research focused on protein engineering and recombinant membrane protein development. Key contributions: • Generated recombinant GPCR constructs for structural biology applications. • Performed molecular cloning, protein expression and purification workflows. • Generated and screened 14 recombinant constructs to identify suitable candidates for structural studies. • Worked with bacterial and insect expression systems. • Applied protein characterization methods including chromatography, electrophoresis and FRAP. • Participated in early-stage membrane protein stabilization and crystallization studies.