Castro Valley, California, United States
Detail-oriented and highly motivated Clinical Genetics Molecular Biology Scientist (CGMBS) professional with 1+ year of Clinical experience and 5+ years of research experience. I am currently seeking opportunities in molecular biology, genetic testing, and laboratory techniques in a challenging and dynamic environment.
• Performed capillary based Fragment analysis assays for B-cell and T- cell clonality lymphoma testing using 3500xl instrumentation. • MYD88 and EGFR testing using Real-time PCR (qPCR) technique. • Pyrosequencing to analyze genetic sequences for MGMT. • RFLP analysis to differentiate between genetics variations of EGFR hot spots. • Experience in manual NGS Library preparation • Performed DNA extraction from blood and bone marrow samples using Qia Symphony. • Participated in CAP proficiency testing
• Diagnosis and Prognosis of Oncology patients using FISH • Bone Marrow Set up and Harvesting • Chromosome Analysis using Ikaros software
• Automated DNA extraction from whole blood and FFPE tissues. • Nested PCR and Sequencing for diagnosis in PKD patients. • MLPA and qPCR for diagnosis in Oncology samples. • Culture and Maintenance of Amnio and CVS sample. • Aneuploidy detection in Prenatal samples using QF-PCR. • Whole Genome analysis in Prenatal samples for Copy number gain and loss using Microarray.
• Maintenance and culture of different cancer cell lines. Generation of stable cell lines using antibiotic selection. • Studied effects of amplitude enhancer compounds on the core clock genes in cancer cell lines. • SHP compounds screening in 3T3-Fib Per2-Luc cell line to find new circadian targets (amplitude enhancers).
Our lab is interested in understanding how receptors involved in antigen recognition initiate signal transduction events that regulate cell responses in the immune system and enables us to explore the role of TCR signal strength in T cell development and T cell activation.