Odder, Central Denmark Region, Denmark
Since I was introduced to fluorescence based flow cytometry in 2001, I am excited to work with this fantastic technical platform. Within academic basic and clinical research environments, I have acquired knowledge and experience in experimental design and implementation, experiment conduction and documentation, as well as data analysis and interpretation. In November 2020, I have entered the world of mass cytometry (heavy metal based flow cytometry). Since then, at the Department of Biomedicine in the Paludan laboratory (Søren Riis Paludan), we have established and implemented this technical platform (CyTOF Helios) for suspension cytometry based experiments. Helios™ is the 3rd generation of the CyTOF® mass cytometer system and designed to analyze up to 50 metal-tagged antibodies per cell, in single cell suspensions. In August 2022, the Hyperion™ Imaging module was installed as add-on to the Helios system, allowing to analyze metal-tagged antibodies in tissue. This enables to stain with 40+ antibodies simultaneously for spatial proteomics at sub-cellular level (1µm2 resolution). The goal to offer high quality mass cytometry service to research groups at the Aarhus university and beyond.
Manager of the mass cytometry unit (AU,DK), offering suspension and imaging mass cytometry (SMC,IMC) service for national and international customers. More info: https://biomed.au.dk/cytof/
Cell sorting and flow cytometry manager and super user. Providing technical and application support and expert advice, as well as holding the overall responsibility for the day-to-day running and maintenance of the flow analysers (BD FACSCanto2) and cell sorters (BD FACSAria2) at the Research Laboratory at the Department of Haematology, Aalborg Hospital, Aalborg University. Research area: B-cell malignancies, Multiple Myeloma, MRD, relapse, personal medicine, drug resistance
Employed full-time as cell sorting and flow cytometry manager, providing technical and application support and expert advice, as well as holding the overall responsibility for the day-to-day running and maintenance of the flow analysers (BD FACSCanto2) and cell sorters (BD FACSAria2). Research area: B-cell malignancies. Note: Aalborg university became independend from Aarhus University since January 2013
Technical: Establishment of a Fluorescent activated cell sorting facility (FACS) in a BSL-1 laboratory enviroment. Research: Mechanisms concerning the haematological cancer diseases with focus on the human B-cell malignancies and associated disorders.
Scientific Researcher & FACS Service Manager Platform: FACSVantage SE cell sorter, located in a BSL-2 enviroment; FACS Calibur Flow analyser. Flow Cytometry applications: : Immunophenotyping, Cell Cycle, Apoptosis, Proliferation, Fluorescent Proteins; cell sorting. Research: Retroviral‐based gene transfer systems (mouse & lentiviral vectors)