Leipzig, Saxony, Germany
I am an ambitious and detail-oriented M.Sc. Molecular Biotechnology student at Hochschule Anhalt, Germany, with a strong academic foundation in molecular biology, cell biology, and biotechnology. My professional experience includes : 👉🏻Research internship at UFZ Leipzig , University of leipzig( Cell and developmental Biology) Total 8 months 👉🏻IDEXX Diagnostik Laboratory (clinical sample management) - Currently working 👉🏻Master's thesis at Department of Virology (University of Leipzig ) 6 Months, where I was working extensively in cell culture, Transcriptomics analysis and molecular biology techniques to establish transcriptomics methods for an organoid-derived chicken intestinal 2D cell model. 👉🏻Research Project at Department of Biosciences and Medical Biology, University of Salzburg, Austria, where I am working with kinetics of F. nucleatum and internalization study in colorectal cancer cell line and immune cells model ( 3 Months) - Funded by European Region Action Scheme for the Mobility of University Students program My expertise spans: ✅ Cell culture & immunoassays – Handling immune cells, flow cytometry, and staining techniques, Hematology and clinical sample preparation and testing; organoid and chicken intestinal cell line ✅ Molecular techniques – DNA/RNA isolation, qPCR, protein analysis (SDS-PAGE, western blot), sequencing, Microbiology and molecular genetics techniques ✅ Bioinformatics & data analysis – Basics of Python and R, FlowJo, FCS Express, and sequencing software With a dual master's degree in Molecular Biotechnology and Biosciences, I am eager to apply my biopharmaceutical research, diagnostics, and biotechnology innovation skills, and compliance with health and safety regulations in the laboratory environment. I thrive in collaborative and problem-solving environments and am driven by the goal of advancing biomedical research and therapeutic solutions
• Conducted in-depth research on the kinetics and internalization of Fusobacterium nucleatum and F. animalis using Caco2 epithelial cell models. • Investigated colorectal cancer-associated cellular alterations through patient-derived 3D gastric organoids. • Performed transcriptomic analysis including RNA isolation, cDNA synthesis, qPCR, SDS-PAGE, and western blotting under BSL2 and BSL1 protocols. • Gained valuable experience in handling various cancer cell lines, contributing to advancements in cancer research.
• Conducted research at the Institute of Virology, Faculty of Veterinary Medicine, focusing on transcriptomic methods. • Developed and validated qPCR methods, including the selection of suitable reference genes. • Performed plasmid isolation, RNA isolation, cDNA synthesis, and 2D cell culture to support research objectives.
• Conducted research on cuticle-associated gene and protein expression in Drosophila melanogaster. • Utilized advanced molecular biology techniques including RNA isolation and confocal microscopy. • Engaged in DNA digestion, qRT-PCR, Western blotting, and SDS-PAGE to enhance understanding of molecular biology. • Collaborated with a team at Leipzig University to contribute to innovative genetic engineering projects.
• Conducted isolation of Peripheral Blood Mononuclear Cells (PBMCs) using density gradient centrifugation to support immunological research. • Cultured immune cells, including T-cells, B-cells, and monocytes, under optimized conditions to ensure experimental accuracy. • Performed multi-color flow cytometry analysis with Cytek Aurora, enhancing high-dimensional immune profiling capabilities.