Leiden, South Holland, Netherlands
Biotechnology professional with an MSc in Molecular Genetics & Biotechnology (Leiden University) and broad 6 years experience of spanning upstream R&D, molecular biology, and GMP environments. Currently at Bristol Myers Squibb, with previous contributions to vaccine development and technology transfer at Janssen Pharmaceuticals.Brings a strong translational mindset—bridging research, process understanding, and quality considerations—to deliver robust, scalable scientific outcomes. Experienced in cross-functional collaboration and mentoring within multidisciplinary teams.SOP writing & execution, documentation practices, EMPQ, and regulatory compliance, with a detail-oriented approach to maintaining inspection-ready environments and supporting quality operations.Authorized to work in the Netherlands (EU-Permanent Residence; no visa sponsorship required).
• Perform environmental monitoring, growth promotion testing, BacT analysis, 3P-Station, and MALDI-TOF identification • Support Environmental Monitoring Performance Qualification (EMPQ) during new cleanroom setup, lifecycle validation activities, and process setups and verifications. • Perform deviation investigations and EM excursions • Draft, review, and update SOPs, Work Instructions, and controlled documentation • Act as GMP trainer ensuring compliance across teams, supporting knowledge transfer and consistent ways of working within the team • Contribute to investigational projects to improve process robustness. Supporting Aseptic Process Simulation (APS), APQ, utility testing, and cleanroom validation • Collaborated cross-functionally with manufacturing, QA and MSAT through microbiological testing, environmental monitoring, APS, and APQs - Certified in Six Sigma White Belt and Green Belt methodologies.
• My work supported antigen production strain development, pre-GMP cell bank preparation, and reference antigen production. • Trained in adhering to the cGMP regulatory guidelines to ensure the quality of seed material. • Key experimental role includes different immunological and biophysical characterisation assays (SDS-PAGE, western blotting, cell viability testing, gram staining, MALDI-TOF analysis, Purity testing, serotyping by agglutination assay etc). • Involved in stock control and sample management. • Involved in writing Electronic lab notebooks and GMP grade documents. • Supported data analysis, reporting and storage (sequence analysis softwares, storage databases softwares). • Support technology transfers of upstream process department to internal and external partners.
Project Title: “Characterization of afr, novel AraC family transcriptional regulator in Pseudomonas Putida S12” • Executed the characterization of a Transcriptional regulator (TR) in P. putida S12 with ChIP-seq experiment & its optimization studies. • Presented in scientific talks and presentations while working on multiple experiments. Techniques used: RT-qPCR, ChIP-seq, genomic DNA extraction, Western blotting, PCR and PCR based cloning using the pEMG-Knockout system
The Project was funded by the Department of Biotechnology, Government of India. Project Title: “To study the biotechnological utilization of methane by methanotrophs” • Supported the upstream process development team. • My responsibilities included the setup of a seed scale and a fermenter of up to 10 liters. • Experienced in communication with a range of stakeholders. • Performed the Isolation and screening of methanotrophs in anaerobic conditions. • Developed experience in protocol optimization and scale-up studies. Techniques used: PCR, genomic DNA extraction, gas fermenting strain handling (Methanotrophs), plasmid preparation technique, gel extraction, and gel electrophoresis.
Internship Title: To study the effect of wild type and engineered MYCOBACTERIOPHAGE D29 holin on the growth and survival of bacteria. Studied the cytotoxic effect of holin genes upon induction in different physiological conditions. Techniques used: Manual plasmid preparation, transformation techniques (Heat-shock method and Electroporation), induction assay, protein purification, growth kinetics study, expression analysis, Western blotting, and SDS-Tricine PAGE.