New York City Metropolitan Area
Experienced research scientist with a demonstrated history of working in the biotechnology industry. Skilled in assay development, cell culture, western blotting and microscopy. Strong research professional with a Master's degree focused in Cellular and Molecular Biology from Rowan University School of Osteopathic Medicine.
Developing and maintaining cell lines expressing various protein therapeutics. Primarily working with a technology development group to enhance host cell efficiency and time to develop new cell lines. • Construct mammalian expression vectors using PCR amplification, gel electrophoresis, cloning, and bacterial transformation to support therapeutic protein production. • Generate monoclonal cell lines through transfection, single cell cloning, and expansion, ensuring high quality, stable expression of target proteins. • Quantify protein expression levels in cell pools and clones using Octet biolayer interferometry to guide clone selection. • Assess gene copy number and transcript abundance in pools and clones using Digital Droplet PCR (ddPCR) to correlate molecular characteristics with productivity.
Research for the discovery and development of small molecule reactivators for mutant p53 in precision oncology. • Generated stable knockout and TET inducible cell lines with lentivirus used in target validation and screening assays. • Developed luciferase reporter assay and screened ~2000 fragments through assay for p53 reactivators. • Validated potential new targets through proliferation assays with tool compounds, Western blots and preproduction of published work.
Drug discovery for the treatment of NAFLD and NASH targeting lipid accumulation and inflammation. • Developed high-throughput screens used for hit selection. • Optimized cell-based reporter assays for hit conformation of small molecules. • Conducted molecular techniques such as western blotting, RT-PCR, and immunofluoresence to identify and characterize new targets. • Analyzed tissue collected from small animal models to determine in vivo efficacy of lead compounds.
Worked alongside licensed pharmacist to provide quality pharmacetautical care in a high-volume retail setting. • Communicated with healthcare providers, insurance companies and patients. • Trained new employees on daily pharmacy procedures as well as NJ Board of Pharmacy, HIPAA, and OSHA regulations. • Maintained pharmacy records in compliance with federal regulations, and assisted with insurance and regulatory audits.
Collaborated with research technicians to develop and study phenotypic changes in knockout stains of Haloferax volcanii used for publication.