Biotechnology Research · Stockholm
The Proteome of Metabolism Group is led by Assistant Professor Florian Rosenberger and was established in 2025 at Karolinska Institutet in Sweden. The lab is part of the Department of Medical Biochemistry and Biophysics and is a member of the Division of Molecular Metabolism at KI. Research Focus We study mitochondrial metabolism across the diverse cell types of the human body, exploring how metabolic networks differ from one tissue to the next in health and disease. Our particular focus is mitochondrial disease and the puzzle of tissue specificity: understanding why genetic defects in ubiquitous metabolic pathways lead to symptoms in only certain organs. Clinical Collaboration We are working to transform how mitochondrial disorders are diagnosed. By connecting proteomic signatures to clinical phenotypes, we aim to move beyond traditional approaches and enable more precise diagnosis. Our goal is to bridge the gap between cutting-edge proteomics and real clinical benefit for patients with metabolic disease. Methods & Technology Our work lies at the intersection of spatial and single-cell proteomics. We develop new methods for single-cell analysis, including improved sample preparation and chromatography workflows to enable high-throughput studies. Through Deep Visual Proteomics (DVP), we add a spatial dimension by combining laser microdissection with mass spectrometry to analyze proteomes at single-cell resolution while preserving tissue context. Mission Our core goal is to translate proteomics insights into clinical impact. By integrating advanced proteomic technologies with clinical data, we aim to improve disease diagnosis and prognosis and to support clinical management strategies for metabolic and mitochondrial disorders.